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KMID : 0355219950200020461
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Journal of Korean Academy Oral Medicine
1995 Volume.20 No. 2 p.461 ~ p.476
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IMMUNOLOGICAL EFFECT OF THE CYTOCHROME P450 TO ALCOHOL AND STRESS IN GUINEA PIG
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Abstract
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The purpose of this study was to investigate the effect of alcohol and stress on liver and buccal mucosa in guinea pig by immunological methods. Especially, Cytochrome P450(CYP) which is oxidase during alcohol metabolism and bioactivator to carcinogen was used as an indicator in this study.
48 guinea pigs were used in this study.
The experimental guinea pig were divided into three groups The first was a group with giving alcohol-15%(v/v) ethyl alcohol, the second group was a with giving stress in the 0°C water and the third was a control group.
Every 4 guinea pigs of each group were sacrified weekly- first, second, third fourth week after experiment and extracted liver tissues and buccal mucosa. The liver tissues were observed by using immunoblotting technique( Western blot) and buccal mucosa were observed by immunofluorescence technique.
The results were as follows ;
1. By the alcohol and stress, Cytochrome P450 was amplified positive in the liver tissues at third week. 2. By the alcohol and stress, Cytochrome P450 was not detected in the buccal mucosa at any period.
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KEYWORD
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